Up Up Girls (2)
- Takahagi Chinatsu (高萩千夏)
- Yoshikawa Mayu (吉川茉優)
- Kajishima Aya (鍛治島彩)
- Hashimura Riko (橋村理子)
- Nakagawa Chihiro (中川千尋)
- Sasaki Honoka (佐々木ほのか)
- Morinaga Niina (森永新菜)
- Niikura Ami (新倉愛海)
- Shimazaki Yuria (島崎友莉亜)
- Nakaoki Rin (中沖凜, graduated December 31, 2018)
An audition for a second generation of Up Up Girls was announced at the Up Up Girls (Kakko Kari) Nippon Budokan concert, though it was not specified if the winners would be members of the group or part of a new sister group. An age limit of 12 to 23 years old was also reported, but starting and ending dates for the audition were not given.
In a surprise announcement via YouTube on March 18th, Nakaoki Rin was announced to join Up Up Girls (2). The five girls debuted on Up Up Girls (Kakko Kari)'s "Upper Disco / FOREVER YOUNG" single. From then on, the second generation would be acting as their own separate group, Up Up Girls (2), with the release of their first indies single on August 15th, titled "Sun!×3 / Ninoashi Dancing".
On April 22nd, Nakagawa Chihiro and Sasaki Honoka were announced as new members of the group. They would make their debut with the group on their fourth indie single "Zenbu Seishun! / Angel Enjite 20nen".
On December 11th, it was announced that Nakaoki Rin would be graduating from the group and retiring from the entertainment industry on December 31st to focus on her education.
On March 3rd, it was announced that Morinaga Niina, Shimazaki Yuria, and Niikura Ami were the winners of the new member auditions. They are to debut with the group on their sixth indie single "We are Winner! / Starting Over" on April 2nd.
- [2017.08.15] Sun!×3 / Ninoashi Dancing (二の足 Dancing; Hesitant Dancing)
- [2018.01.01] Doshaburi no Terrace Seki / Fuyu Tote Tote (どしゃぶりのテラス席 / フユトテトテ)
- [2018.05.01] Tte Iu Hatsukoi no Oyakusoku / Happiece LOVE♡ (っていう初恋のお約束 / ハッピースLOVE♡)
- [2018.08.07] Zenbu Seishun! / Angel Enjite 20nen (全部青春! / エンジェル演じて20年)
- [2018.12.25] Kakatte Kinasai / OVER DRIVE (かかって来なさい / OVER DRIVE)
- [2019.04.02] We are Winner! / Starting Over (We are Winner! / スターティングオーバー)